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CLM77K / CLM77Ki Cryo Stages
        Instec’s CLM77K is a specialized stage designed for use on any upright light microscope for correlative cryo-light and cryo-electron microscopy (CLEM). The specially designed grid holder allows up to nine 3 mm EM grids to be imaged via bright field and fluorescence light microscopy for determination of regions and/or cellular events of interest for later observation in the electron microscope. The grid holder is designed to keep the grids vitreous - well below the amorphous ice phase transition temperature - during transfer of grids into the grid holder and then into the CLM77K stage. To eliminate frost build up on samples, the chamber can be gas purged and the grid holder has an integrated slide cover that is closed during transfer from liquid nitrogen into the CLM77K chamber. The CLM77Ki is also available for inverted microscopes.
Features
• Programmable precision temperature from -190°C to 120°C
• Swing cover for easy sample access
• Specially designed cryo grid sample holder, capable of holding up to nine grids
• Grid holder remains chilled during transfers
• Integrated slide cover on grid holder to eliminate moisture condensation on samples
• Removable and exchangeable windows
• Dual pane windows for better thermal isolation
• Integrated aperture window defrost system
• Gas purge sample chamber
• Easy side sample loading with standard microscope slides
• Vertical and horizontal mounting
• Integrated precision X-Y micropositioner for in situ sample positioning
• Optional microscope stage mounting adapters available
Technical Specifications
Temperature Range
-190°C to 120°
The LN2-SYS liquid nitrogen cooling system is included with the CLM77K
Temperature Resolution
0.01°C with mK2000
Temperature Stability
±0.05°C at 100°C with mK2000 (better stability optional)
Minimum Heating and Cooling Rate
±0.1°C per hour
Maximum Heating Rate
+60°C per minute at 100°C
Maximum Cooling Rate
-50°C per minute at 100°C (when using LN2-P4)
Temperature Control Method
Switching PID
Temperature Control Sensor
100 Ω Platinum RTD
Minimum Objective Working Distance
5 mm
Minimum Condenser Working Distance
17.6 mm (CLM77K),19.3mm (CLM77Ki)
Grid Holder
Holds nine 3 mm diameter sample grids
Sample Viewing Aperture
3 mm diameter
X-Y Micropositioner
10 μm resolution
Weight 1,280 g (CLM77K), 1,070 g (CLM77Ki)
 
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